Rat Kidney Epithelial Cells. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate while allowing other substances of no nutritional value to be excreted in the urine. These cells exhibit aldosterone stimulated transepithelial na currents that depend on both the apical epithelial na channel enac and basolateral na k atpase.
Male rat kangaroo kidney epithelial cells ptk2 the widely used ptk2 cell line was established from the kidney tissue of an adult male rat kangaroo potorous tridactylus a long nosed marsupial that is fairly common in australia. Balb c mouse primary kidney epithelial cells from cell biologics are isolated from kidney tissue of pathogen free laboratory mice. The nrk cell line was derived from the kidney of a rat of the species rattus norvegicus.
Expression of epithelial na channel enac protein in the apical membrane of rat kidney tubules was assessed by biotinylation of the extracellular surfaces of renal cells and by membrane fractionation.
Renal proximal tubular epithelial cells rptepic play a crucial role in renal function. Tissue culture reagents including dulbecco s modified eagle s medium dmem calf serum trypsin penicillin streptomycin protease inhibitor cocktail cdcl 2 and all other chemicals and biological reagents were obtained from sigma st. Epithelial cells are cells that exist in the body in sheets covering the organs and other internal and external surfaces that may come into contact with foreign materials. The nrk cell line was derived from the kidney of a rat of the species rattus norvegicus.