Kidney Brush Border Enzymes. Clinically it has proven that the proximal tubule of the kidney is especially susceptible to ischemic inflammatory or toxic events. Meprin is cytotoxic to cultured renal tubular.
The brush border marker enzymes alkaline phosphatase and aminopeptidase m were found to be enriched 17 0 5 3 fold and 16 7 3 7 fold respectively. 47 0 9 5 for aminopeptidase m. Compared with the same fab radioiodinated by the direct electrophilic method the construct containing the brush border enzyme cleavable linker exhibited a 6 fold decrease in kidney uptake in athymic mice at 3 h while no decrease in.
Background it has been shown that non congenic mice strains with lower levels of renal meprin develop less renal injury following renal ischemia and reperfusion.
47 0 9 5 for aminopeptidase m. The microvilli that constitute the brush border have enzymes for this final part of digestion anchored into their apical plasma membrane as integral membrane proteins. Background it has been shown that non congenic mice strains with lower levels of renal meprin develop less renal injury following renal ischemia and reperfusion. The specific activities of the enzymes were decreased several hundredfold in the brush border preparation compared with a kidney homogenate but the enzymes were not totally absent.