Human Fetal Kidney Epithelial Progenitor Cells. Epithelial cell marker expression in a 20 week fetal human trachea implanted 15 weeks in the scid mouse. A mature pseudostratified mucociliary epithelium lines the whole inner surface of the graft and rests on a thick mesenchyme.
Cell reports article dissecting the global dynamic molecular profiles of human fetal kidney development by single cell rna sequencing ping wang 1 2 6 yidong chen 1 3 4 6 jun yong 1 4 yueli cui 1 2 rui wang 1 2 lu wen 1 2 jie qiao 1 3 4 5 and fuchou tang1 2 3 7 1beijing advanced innovation center for genomics department of obstetrics and gynecology third hospital college of life science. The fetal kidney progenitor cells express the mrna encoding the embryonic kidney mesenchymal epithelial transcription factor pax 2 dressler and douglass 1992 the stem cell surface antigen protein cd133 miraglia et al 1997 which is expressed in embryonic kidney corbeil et al 2000 the kidney embryonic progenitor marker protein cd24 challen et al 2004 as well as cd29 and cd44 proteins. Human embryonic kidney 293 cells also often referred to as hek 293 hek 293 293 cells or less precisely as hek cells are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture taken from an aborted female fetus.
Analysis of single cell sequencing data of 6414 cells from five human embryonic kidneys revealed 11 clusters fig.
Cell reports article dissecting the global dynamic molecular profiles of human fetal kidney development by single cell rna sequencing ping wang 1 2 6 yidong chen 1 3 4 6 jun yong 1 4 yueli cui 1 2 rui wang 1 2 lu wen 1 2 jie qiao 1 3 4 5 and fuchou tang1 2 3 7 1beijing advanced innovation center for genomics department of obstetrics and gynecology third hospital college of life science. The cultures appear to represent early kidney epithelial progenitors based on cellular marker expression. Therefore the combination of these two markers is not likely to enrich a renal progenitor phenotype. Definition of useful markers is crucial for the identification of hfk stem cells.